HPLC Run Decision Tool
Set and validate the method specifications, then upload CSV or XLSX exports. Valid runs receive a next-run recommendation.
Method Specifications
| Target No. Compounds/Peaks | Maximum Run Time (min) | Resolution (Rmin) | Max Back-pressure (psi) |
|---|---|---|---|
The decision engine has been paused because one or more values are invalid. Correct the listed specifications and run the analysis again.
Ambient run: Set Temp is 25°C. Measured Temp does not determine whether a run is ambient.
Upload a run
No file selected
Uploaded run records are saved in this browser.
Column & Flow Parameters
These bookkeeping values are read from the uploaded run where available.
Inconsistencies Found in Naming: Manually Edit Column & Flow Parameters For accurate data recognition
Dashboard
Correct the specifications above, then validate and run the analysis again.
| Group No. | Trial No. | Ligand | Column Type | Coreshell | Pore (Å) | C-Load (%) | Length (mm) | ID (mm) | Particle (μm) | Solvent | %B | Flow (mL/min) | Conc. (mg/mL) | Sample Volume (µL) | Set Temp (°C) | Measured Temp (°C) | Wavelength (nm) | Decision | Actions | Peaks | Last-peak retention (min) | Lowest Rs (Rmin) | Back-pressure (psi) |
|---|
Extraction log
Upload a run to see extracted peaks, width thresholds, and calculated values.
HPLC Modules Guide
Choose an HPLC module, then select one of its parameters. The guide explains what it controls, how it can affect retention, selectivity, and efficiency, and whether this decision tool recommends changing it.